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Figure 1 Injection of different concentrations of human PLC cRNA causes Ca2+ oscillations in aged human oocytes. Shown are three sample traces of the intracellular Ca2+ concentration, as measured by Oregon Green BAPTA dextran fluorescence (Fl.; in arbitrary units). Recordings were made from aged human oocytes after injection of PLC cRNA. In each panel the oocytes were injected 1020 min before the start of the recording. The concentrations to the right of the traces refer to those in the injection pipette (25% oocyte volume injected). The repetitive rises in Ca2+ are seen as distinct spike-like increases in fluorescence.
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