This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Kane, M. T. Articles by Harrison, R. A. P. Search for Related Content PubMed Articles by Kane, M. T. Articles by Harrison, R. A. P.

Uptake and incorporation of inositol by preimplantation mouse embryos

Summary. The uptake of myo-inositol by preimplantation mouse embryos was investigated using [³H]myo-inositol. Uptake increased about 12-fold between one- and two-cell stages and increased again at the blastocyst stage (> 6-fold compared with the two-cell stage). Uptake at the blastocyst stage was time and temperature dependent; it was stimulated by sodium, inhibited by glucose and appeared to take place mainly via a saturable mechanism. Uptake in the presence of 6·25 mmol inositol l^–1 was 1424 fmol inositol per blastocyst per h. About 10% of the [³H]inositol taken up by blastocysts during 8 h in culture was incorporated into lipid. Thin layer chromatography of the lipid showed that most of this inositol was incorporated into lipid material co-migrating with phosphatidylinositol with a small proportion co-migrating with phosphatidylinositol 4-phosphate.

Keywords: mouse; embryo; inositol; phosphoinositides