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Journal of Reproduction and Fertility (1992) 95 263-268
DOI: 10.1530/jrf.0.0950263
Copyright © 1992 Society for Reproduction and Fertility
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Effect of different fractions of seminal plasma on the fertilizing ability of fowl spermatozoa stored in vitro

E. Blesbois and M. de Reviers

Summary. This paper describes the effects of whole seminal plasma and of dialysed seminal plasma on the fertilizing ability of fowl spermatozoa stored for 24 h at 4°C. The fertilizing ability of fowl semen diluted 1:1 with Beltsville Poultry Semen Extender and stored for 24 h at 4°C was enhanced after replacement of the homologous seminal plasma by the diluent (89 versus 77% fertilization rate). Better results were obtained with seminal plasma dialysed against water before sperm storage to discard the < 1 kDa or the < 50 kDa fractions. It was concluded that low molecular weight seminal plasma fractions could damage the fertilizing ability of spermatozoa during storage at 4°C, whereas high molecular weight fractions appeared to enhance fertilizing ability.

Keywords: seminal plasma; fertilizing ability; fowl







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Copyright © 1992 by the Society for Reproduction and Fertility.