This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hyttel, P. Articles by Greve, T. Search for Related Content PubMed Articles by Hyttel, P. Articles by Greve, T.

Ultrastructure of in-vitro oocyte maturation in cattle

Summary. Cumulus–oocyte complexes were collected from cows at an abattoir by aspiration from small (1–6 mm) antral follicles. After different periods of culture the complexes were processed for electron microscopy. Cumulus expansion occurred at 12–18 h of culture and concomitantly enlargement of cumulus cell projections in the perivitelline space was seen. At 48 h the innermost cumulus cells flattened and adhered tightly to the zona pellucida. In the oocyte the following changes occurred: at 0–3 h of culture the perivitelline space developed; at 3–12 h disconnection of the junctions between cumulus cell projections and the oolemma, and the concomitant breakdown of the nucleus was observed; at 12–18 h the mitochondria moved from a peripheral location to a more even spatial distribution and the Golgi complexes decreased in size; at 18 h the smooth endoplasmic reticulum formed large aggregates surrounded by mitochondria; at 18–21 h the first polar body was abstricted; at 24–40 h the cortical granules spread; at 30–40 h the polar body degenerated in some specimens; at 40–48 h the perivitelline space decreased in size; and at 48 h one oocyte was in the process of fragmentation. It is concluded that nuclear and cytoplasmic oocyte maturation is simulated in vitro. However, certain deviations were noticed compared to in-vivo maturation.

This article has been cited by other articles:

				G. E. Schrock, A. M. Saxton, F. N. Schrick, and J. L. Edwards Early In Vitro Fertilization Improves Development of Bovine Ova Heat Stressed During In Vitro Maturation J Dairy Sci, September 1, 2007; 90(9): 4297 - 4303. [Abstract] [Full Text] [PDF]

				R G Sturmey, P J O'Toole, and H J Leese Fluorescence resonance energy transfer analysis of mitochondrial:lipid association in the porcine oocyte. Reproduction, December 1, 2006; 132(6): 829 - 837. [Abstract] [Full Text] [PDF]

				D. Nogueira, R. Ron-El, S. Friedler, M. Schachter, A. Raziel, R. Cortvrindt, and J. Smitz Meiotic Arrest In Vitro by Phosphodiesterase 3-Inhibitor Enhances Maturation Capacity of Human Oocytes and Allows Subsequent Embryonic Development Biol Reprod, January 1, 2006; 74(1): 177 - 184. [Abstract] [Full Text] [PDF]

				A. Ali and M.-A. Sirard Effect of the Absence or Presence of Various Protein Supplements on Further Development of Bovine Oocytes During In Vitro Maturation Biol Reprod, April 1, 2002; 66(4): 901 - 905. [Abstract] [Full Text] [PDF]

				D. Viuff, P. J.M. Hendriksen, P. L.A.M. Vos, S. J. Dieleman, B. M. Bibby, T. Greve, P. Hyttel, and P. D. Thomsen Chromosomal Abnormalities and Developmental Kinetics in In Vivo-Developed Cattle Embryos at Days 2 to 5 after Ovulation Biol Reprod, July 1, 2001; 65(1): 204 - 208. [Abstract] [Full Text] [PDF]

				T. Fair, P. Hyttel, P. Lonergan, and M. P. Boland Immunolocalization of Nucleolar Proteins During Bovine Oocyte Growth, Meiotic Maturation, and Fertilization Biol Reprod, May 1, 2001; 64(5): 1516 - 1525. [Abstract] [Full Text]

				M. Asada, M. Horii, T. Mogoe, Y. Fukui, H. Ishikawa, and S. Ohsumi In Vitro Maturation and Ultrastructural Observation of Cryopreserved Minke Whale (Balaenoptera acutorostrata) Follicular Oocytes Biol Reprod, February 1, 2000; 62(2): 253 - 259. [Abstract] [Full Text]