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The concerted effect of -chlorohydrin and glucose on the ATP concentration in spermatozoa is associated with the accumulation of glycolytic intermediates

Summary. In the absence of a glycolysable sugar the effect of 1 mM-RS--chlorohydrin on the ATP concentration in ram or boar spermatozoa was relatively small but the addition of 0·10 or 0·03 mM-glucose initiated a rapid loss of ATP. When the spermatoza were incubated with 0·05mM-RS--chlorohydrin, the addition of 1·0mM (ram) or 0·06 mM (boar)-glucose was required to produce ATP dissipation. In ram spermatozoa treated with 0·05 or 1·00mM-RS--chlorohydrin, ATP loss was caused by 10 mM-fructose or 10mM-mannose but not by 10mM-glycerol or 10mM-inositol. In boar spermatozoa incubated with 1 mM-RS--chlorohydrin the addition of 10mM-L-lactate plus 1·0 mM-pyruvate protected the spermatozoa against the ability of 1·0 mM-glucose to produce a decline in ATP concentration.

Every combination of treatments capable of inducing a marked decline in ATP concentration also caused a dramatic (20–100-fold) increase in the concentration of fructose 1,6-bisphosphate. An increase in fructose 1,6-bisphosphate concentration was never observed when the ATP concentration was unaffected. We conclude that it is very probable that the concerted effect of a-chlorohydrin and glycolysable sugar is responsible for the contraceptive action of -chlorohydrin in vivo and that fructose 1,6-bisphosphate is implicated in its mechanism of action.

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