This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Le Magueresse, B. Articles by Jégou, B. Search for Related Content PubMed PubMed Citation Articles by Le Magueresse, B. Articles by Jégou, B.

Stimulation of rat Sertoli cell secretory activity in vitro by germ cells and residual bodies

Summary. The direct influence of germ cells and residual bodies on Sertoli cell basal and FSH-stimulated secretion of androgen-binding protein (ABP) was studied using Sertoli cells, recovered from 20-day-old rats, cultured alone or cocultured with a crude germ cell preparation from adult rats or with pachytene spermatocytes, round spermatids or populations of residual bodies enriched by centrifugal elutriation. The effect of a rat liver epithelial cell line (LEC) on Sertoli cell function was also tested. Addition of a crude germ cell preparation increased basal and FSH-stimulated ABP secretion. Pachytene spermatocytes and residual bodies adhered to the Sertoli cell monolayer to a much greater extent than did round spermatids. Addition of pachytene spermatocytes markedly enhanced basal and FSH-stimulated ABP secretion over 12 days of culture. Round spermatids and residual bodies stimulated ABP secretion although to a lesser extent than did spermatocytes. Furthermore, the increase of FSH-stimulated ABP levels was not maintained after 4 or 8 days of culture. LEC also enhanced basal and FSH-induced ABP levels but the increase of FSH-induced ABP production was only observed until Day 8 of culture. The influence of LEC on Sertoli cell secretion could be mediated through the production of an extracellular matrix. It is concluded that germ cells, particularly pachytene spermatocytes, can directly stimulate Sertoli cell secretory activity in vitro.

This article has been cited by other articles:

				V. Roulet, H. Denis, C. Staub, A. Le Tortorec, B. Delaleu, A.P. Satie, J.J. Patard, B. Jegou, and N. Dejucq-Rainsford Human testis in organotypic culture: application for basic or clinical research Hum. Reprod., June 1, 2006; 21(6): 1564 - 1575. [Abstract] [Full Text] [PDF]

				J. Longin, P. Guillaumot, M.-A. Chauvin, A.-M. Morera, and B. Le Magueresse-Battistoni MT1-MMP in rat testicular development and the control of Sertoli cell proMMP-2 activation J. Cell Sci., January 6, 2001; 114(11): 2125 - 2134. [Abstract] [Full Text] [PDF]

				J. Blanco-Rodríguez and C. Martínez-García Apoptosis Is Physiologically Restricted to a Specialized Cytoplasmic Compartment in Rat Spermatids Biol Reprod, December 1, 1999; 61(6): 1541 - 1547. [Abstract] [Full Text]

				B. Le Magueresse-Battistoni, G. Pernod, F. Sigillo, L. Kolodié, and M. Benahmed Plasminogen Activator Inhibitor-1 Is Expressed in Cultured Rat Sertoli Cells Biol Reprod, July 1, 1998; 59(3): 591 - 598. [Abstract] [Full Text]

				B. Le Magueresse-Battistoni, G. Pernod, L. Kolodie, A.-M. Morera, and M. Benahmed Tumor Necrosis Factor-{alpha} Regulates Plasminogen Activator Inhibitor-1 in Rat Testicular Peritubular Cells Endocrinology, March 1, 1997; 138(3): 1097 - 1105. [Abstract] [Full Text] [PDF]

				N Gerard, A Corlu, B Kneip, H Kercret, M Rissel, C Guguen-Guillouzo, and B Jegou Liver-regulating protein (LRP) is a plasma membrane protein involved in cell contact-mediated regulation of Sertoli cell function by primary spermatocytes J. Cell Sci., January 3, 1995; 108(3): 917 - 925. [Abstract] [PDF]

				J. J. Heindel and K. A. Treinen Physiology of the Male Reproductive System: Endocrine, Paracrine and Autocrine Regulation Toxicol Pathol, February 1, 1989; 17(2): 411 - 445. [Abstract] [PDF]