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Serotonin-induced stimulation of progesterone production by cow luteal cells in vitro

Summary. The addition of acetylcholine or histamine (10^{– 7} to 10^–4 M), -aminobutyric acid, a dopamine agonist, and melatonin (10^–7 to 10^–5M) did not alter basal or LH-stimulated progesterone production (P>0.05). The addition of the specific β₂-adrenergic agonist terbutaline and salbutamol did not significantly elevate progesterone production. Treatment of luteal cells with serotonin (5-HT), 10^–6 to 10^–4 M, increased the production of progesterone (P<0.05). This stimulated production was inhibited by the addition of mianserin (10^–5M, a 5-HT antagonist; P<0.05). Isoproterenol (10^–7 to (10^–4M) also resulted in significant increases in progesterone production (P<0.05). The combined treatments of 5-HT + LH, isoproterenol+ LH, or isoproterenol + 5-HT did not result in a further increase in progesterone above that observed in response to LH or isoproterenol alone (P>0.05). The isoproterenol-induced progesterone production could not be blocked by butoxamine (10^–5M, a β₂-antagonist), or practolol (10^–5M, a β₁-antagonist), but was inhibited by propranolol (10^–5 M, a general β-antagonist; P<0.05). The response to isoproterenol was unaffected by mianserin (10^–5 M). These results demonstrate a possible role for 5-HT in the regulation of steroidogenesis by the corpus luteum of the cow. Furthermore, these results suggest that serotonin-induced progesterone production is a receptor-mediated event.

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