This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zwierzchowski, L. Articles by Guszkiewicz, A. Search for Related Content PubMed Articles by Zwierzchowski, L. Articles by Guszkiewicz, A.

Effect of polyamine limitation on DNA synthesis and development of mouse preimplantation embryos in vitro

L. Zwierzchowski, M. Czonkowska and A. Guszkiewicz

Summary. In-vitro treatment of preimplantation mouse embryos with spermine and spermidine biosynthesis inhibitor, methylglyoxal-bis-(guanylhydrazone) (MGBG), arrested embryo development at the 8-cell or morula stage. In addition, the embryo DNA synthetic rate, as measured by [³H]thymidine incorporation, was strongly inhibited. The inhibition of blastocyst formation and DNA synthesis by MGBG was readily reversible by an exogenous supply of spermine and/or spermidine to the culture medium. DL--Methylornithine or DL--difluoromethylornithine (-DFMO), inhibitors of putrescine biosynthesis, had no effect on embryos cultured for 1 or 2 days, but on the 3rd day embryo DNA synthesis was significantly depressed in the presence of -DFMO. These observations suggest that, during early development of the pre-implantation mouse embryo, spermine and spermidine are involved in regulation of embryo growth and DNA synthesis. They may also indicate a role of putrescine at a later stage of mouse embryo development.

This article has been cited by other articles:

				H. Pendeville, N. Carpino, J.-C. Marine, Y. Takahashi, M. Muller, J. A. Martial, and J. L. Cleveland The Ornithine Decarboxylase Gene Is Essential for Cell Survival during Early Murine Development Mol. Cell. Biol., October 1, 2001; 21(19): 6549 - 6558. [Abstract] [Full Text] [PDF]

				L. J. Van Winkle Amino Acid Transport Regulation and Early Embryo Development Biol Reprod, January 1, 2001; 64(1): 1 - 12. [Abstract] [Full Text]