This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jackson, N. C. Articles by Lendon, R. G. Search for Related Content PubMed Articles by Jackson, N. C. Articles by Lendon, R. G.

Study using in-vivo binding of ¹²⁵I-labelled hCG, light and electron microscopy of the repopulation of rat Leydig cells after destruction due to administration of ethylene-1,2-dimethanesulphonate

Summary. Gonadotrophin binding to rat Leydig cells after a single administration of ethylene dimethanesulphonate (EDS) (75mg/kg i.p.) was followed by using intratesticular microdoses of ¹²⁵I-labelled hCG, whilst corresponding morphological changes in the testicular interstitium were studied with light and electron microscopy. No discernible effect on ¹²⁵I-labelled hCG binding compared with controls was observed until 24 h after treatment. Between 24 and 32 h a sharp decline in binding occurred which was correlated with extensive Leydig cell destruction. By 48 h the ¹²⁵I-labelled hCG binding was negligible and no morphologically recognizable Leydig cells were found at this time. The specific binding remained low until 21 days after treatment and then a marked increase occurred to give nearly normal levels by 49 days. This was consistent with a generalized repopulation of the interstitium with Leydig cells, seemingly the result of differentiation of fibroblast-like precursor cells.

This article has been cited by other articles:

				E. Prabagaran, U. C. Hegde, S. B. Moodbidri, A. H. Bandivdekar, and V. P. Raghavan Postnatal Expression and Androgen Regulation of HOXBES2 Homeoprotein in Rat Epididymis J Androl, September 1, 2007; 28(5): 755 - 771. [Abstract] [Full Text] [PDF]

				J. Tesarik and C. Mendoza Using the Male Gamete for Assisted Reproduction: Past, Present, and Future J Androl, May 1, 2003; 24(3): 317 - 328. [Full Text] [PDF]

				M. F. Taylor, M. de Boer-Brouwer, I. Woolveridge, K. J. Teerds, and I. D. Morris Leydig Cell Apoptosis after the Administration of Ethane Dimethanesulfonate to the Adult Male Rat Is a Fas-Mediated Process Endocrinology, August 1, 1999; 140(8): 3797 - 3804. [Abstract] [Full Text]

				K. J. Teerds, M. de Boer-Brouwer, J. H. Dorrington, M. Balvers, and R. Ivell Identification of Markers for Precursor and Leydig Cell Differentiation in the Adult Rat Testis Following Ethane Dimethyl Sulphonate Administration Biol Reprod, June 1, 1999; 60(6): 1437 - 1445. [Abstract] [Full Text]

				I. Woolveridge, M. de Boer-Brouwer, M. F. Taylor, K. J. Teerds, F. C.W. Wu, and I. D. Morris Apoptosis in the Rat Spermatogenic Epithelium Following Androgen Withdrawal: Changes in Apoptosis-Related Genes Biol Reprod, February 1, 1999; 60(2): 461 - 470. [Abstract] [Full Text] [PDF]