This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ahuja, K. K. Articles by Bolwell, G. P. Search for Related Content PubMed Articles by Ahuja, K. K. Articles by Bolwell, G. P.

Probable asymmetry in the organization of components of the hamster zona pellucida

Summary. The distribution of the sperm-, lectin- and antibody-binding components was examined on isolated fragments of the hamster zona pellucida. In all three studies, binding was much stronger on the outer than on the inner surface of the zona pellucida. Fab antibody-binding sites were different from those for various lectins since there appeared to be no competition for binding. Multiple components are obviously exposed on the surfaces of the zona pellucida because the sperm receptor sites are also inaccessible to Fab fragments. We conclude that although the components may be present throughout the zona they are asymmetrically arranged in relation to both surfaces.

Microdisc PAGE of zonae from ovulated eggs revealed three major components of molecular weight 240 000, 150 000 and 80 000. These are probably glycoproteins and thus resemble the glycoprotein composition of the mouse zona.

This article has been cited by other articles:

				M. Jimenez-Movilla, M. Aviles, M. J. Gomez-Torres, P. J. Fernandez-Colom, M. T. Castells, J. de Juan, A. Romeu, and J. Ballesta Carbohydrate analysis of the zona pellucida and cortical granules of human oocytes by means of ultrastructural cytochemistry Hum. Reprod., August 1, 2004; 19(8): 1842 - 1855. [Abstract] [Full Text] [PDF]