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Measurement of anionic sites of rat epididymal spermatozoa using tritiated polycationized ferritin

Summary. Anionic sites of rat epididymal spermatozoa were measured at pH 7·4 using tritiated polycationized ferritin. The spermatozoa from the caput region had 1·25 ± 0·06 x 10⁶ anionic sites per cell and a binding constant of 1·26 ± 0·01 x 10⁶ M^–1. Spermatozoa from the cauda region had 1·50 ± 0·09 x 10⁶ anionic sites per cell and a binding constant of 4·84 ± 0·82 x 10⁶ M^–1. The values were mean ± s.d. The anionic sites were partly sensitive to treatments with neuraminidase, trypsin and Triton X-100.