This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ford, W. C. L. Articles by Harrison, A. Search for Related Content PubMed Articles by Ford, W. C. L. Articles by Harrison, A.

The activity of glyceraldehyde 3-phosphate dehydrogenase in spermatozoa from different regions of the epididymis in laboratory rodents treated with -chlorohydrin or 6-chloro-deoxyglucose

Summary. The activity of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (mUnits/10⁶ spermatozoa: mean ± s.e.m., N = 12) in spermatozoa from the rat epididymis declined from 22·0 ± 1·4 in the caput to 14·1 ± 1·3 in the corpus region but there was no further decrease in the cauda region. In hamsters (N = 4), GAPDH activity in spermatozoa declined from 24·8 ± 2·2 in the caput to 15·2 ± 1 ·2 in the distal cauda epididymidis with the greatest decrease between the corpus and proximal cauda regions. In guinea-pigs (N = 4) GAPDH activity in spermatozoa increased from 11·4 ± 0·79 in the caput to 18·0 ± 0·7 in the corpus and cauda regions of the epididymis. The activity of GAPDH in spermatozoa therefore changes during maturation in a species dependent manner.

GAPDH in spermatozoa from the distal cauda epididymidis of rats given -chlorohydrin (4, 8 or 25 mg/kg/day by mouth) or 6-chloro-6-deoxyglucose (24 or 96 mg/kg/day by mouth) for 10 days was inhibited by > 80% but was only inhibited by 25– 45% in spermatozoa from the caput epididymidis. The enzyme was inhibited to an intermediate and dose-dependent extent in spermatozoa from the corpus region. A similar pattern of inhibition was seen in spermatozoa from hamsters given -chlorohydrin (50 or 100 mg/kg/day) for 10 days. -Chlorohydrin (66 mg/kg/day s.c.) for 10 days inhibited GAPDH in spermatozoa from the caput or corpus epididymidis of the guinea-pig by <20% but decreased GAPDH activity by almost 90% in the cauda region. In rats the greater effect of -chlorohydrin on spermatozoa from the cauda region of the epididymis occurred even after short periods of treatment or when the passage of spermatozoa through the duct was interrupted by a ligature around the corpus region, indicating that the effect is not simply a reflection of the length of time the spermatozoa have spent in the epididymis. It is concluded that either spermatozoa undergo a maturational change which increases their sensitivity to -chlorohydrin or that -chlorohydrin (or an active metabolite) is concentrated in the lumen of the cauda epididymidis.

This article has been cited by other articles:

				P.-h. Hung, M. G. Miller, S. A. Meyers, and C. A. VandeVoort Sperm Mitochondrial Integrity Is Not Required for Hyperactivated Motility, Zona Binding, or Acrosome Reaction in the Rhesus Macaque Biol Reprod, August 1, 2008; 79(2): 367 - 375. [Abstract] [Full Text] [PDF]