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Bioassay for anti-chorionic gonadotrophin sera

Summary. The method for a rapid bioassay for the neutralizing activities of antichorionic gonadotrophin sera is based on the inhibition of the increase in plasma testosterone concentrations in male mice after the injection of antiserum and human chorionic gonadotrophin (hCG). The antisera were obtained from rhesus monkeys immunized against the beta-subunit of ovine luteinizing hormone (oLHβ). The anti-oLHβ sera neutralized the hCG-induced testosterone stimulation. A dose–response relationship for neutralization was found between 25 and 200 µl antiserum. Anti-hCG sera raised in human, chimpanzee and rabbit also neutralized the biological activity of hCG. The major advantage of this method is that the single injection of antiserum given before hCG administration leads to the hormone–antibody reaction in vivo.