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In-vitro prostanoid production by the rat vas deferens

Summary. Prostaglandins (PGs), E-2, F-2, 6-keto-F-1 and thromboxane B-2 (TXB-2), produced in vitro by the vas deferens of rats at different stages of sexual maturation (10, 35 and 100 days of age), were estimated by radioimmunoassay. Reversed-phase high-performance liquid chromatography was used to evaluate the RIA and to give a more complete profile, after incubation of the vas deferens with [¹⁴C]arachidonic acid. The amount of PGE-2 released into the medium after incubation at 37°C for 5 min increased with age, and was the predominant prostanoid in the adult vas deferens. In prepubertal organs, 6-keto-PGF-1 predominated. TXB-2 was always a minor product. The addition of exogenous arachidonic acid (10 µg/ml/20 mg tissue), provoked a higher production of the four compounds. PGE-2 and PGF-2 levels were reduced after castration or hypophysectomy and were re-instated after treatment with testosterone propionate. PGE-2 was much more sensitive to hormonal deprivation than PGF-2. The production of 6-keto-PGF-1 was not significantly affected by the above treatments.

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