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Effects of passive immunization against oestradiol on mouse ovum transport

Summary. The ability of anti-oestradiol immunoglobulin to withhold oestradiol-17β from its target tissue was examined. The total oestradiol-17β binding capacity present in in-vitro incubations or injected into mice intravenously was related to the amount of [³H]oestradiol present in the media or intravenously injected into the animals respectively. When the ratio of binding capacity to [³H]oestradiol was above 74:1, [³H]oestradiol was successfully withheld from uterine tissue in vitro and in vivo.

Injecting anti-oestradiol immunoglobulin into mice before administration of a tubelocking dose of oestradiol-17β ensured normal passage of ova through the oviduct. Daily administration of anti-oestradiol immunoglobulin to PMSG–hCG stimulated mice (starting 72 h before hCG injection) induced retention of ova for at least 2 days beyond the time when all ova had left the oviducts of control animals. The binding capacity: oestradiol-17β concentration ratios of sera from these animals were >250:1 throughout the experimental period. Non-specific immunoglobulin had no such effects, indicating the specificity of the anti-oestradiol immunoglobulin response.