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Direct assay of bound sialic acids on rat spermatozoa from the caput and cauda epididymidis

Summary. Bound sialic acids on rat spermatozoa were assayed by oxidation with 1 mMNaIO₄ at 0°C, liberating C-9 as formaldehyde which was further quantitated using 3-methyl-2-benzothiazolinone. The mean ± s.d. (n = 20) content of bound sialic acids of spermatozoa from the caput and cauda epididymidis was 50·9 ± 8·0 and 25·2 ± 3·8 nmol/10⁸ spermatozoa respectively. About 85% of the former and 75% of the latter could be extracted by 1% Triton X-100 and 2 mM-dithiothreitol. About 70% of the former and 20% of the latter were released by neuraminidase from Vibrio cholerae. About 40% of the former and 30% of the latter were sensitive to trypsin. During sperm maturation, the decrease in the total bound sialic acids was due to the decrease in the neuraminidase-sensitive but not the neuraminidase-resistant sialic acids.

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