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Spermatozoa of the Atlantic bottlenosed dolphin, Tursiops truncatus

Summary. Semen from a male dolphin in captivity was collected by electroejaculation and frozen to –176°C. Sperm motility was excellent after thawing 10 days later. Electron microscopy showed 14–16 parallel ridges in the post-acrosomal region and two types of mitochondria in the mid-piece. The spermatozoa were capable of fusing with zona-free hamster eggs only after preincubation for 2 h, suggesting the need for sperm capacitation and acrosome reaction before fertilization in this species.

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				T.R. Robeck and J.K. O'Brien Effect of Cryopreservation Methods and Precryopreservation Storage on Bottlenose Dolphin (Tursiops truncatus) Spermatozoa Biol Reprod, May 1, 2004; 70(5): 1340 - 1348. [Abstract] [Full Text] [PDF]