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Two preparations for the study of the isolated rabbit oviduct

Summary. When rabbit oviducts were slit open along their length, cut into small pieces, and incubated in Krebs–Ringer–bicarbonate medium, the tissue slices took up O₂ at a steady rate for 40 min, retained approximately 70% of their adenine nucleotides, and were suitable for metabolic studies of the oviduct. In a second preparation, oxygenated Krebs–Ringer–bicarbonate medium was recirculated at 700 µl/min through the lumen of the whole oviduct, severed of its blood supply, while the serosal surface was bathed in a similar medium. The preparation took up O₂, maintained a steady potential difference across its mucosal and serosal surfaces, and transported 2-deoxy D-glucose selectively for at least 40 min. In oviducts taken from rabbits injected 3 days previously with hCG, there was > 4-fold increase in the initial transmural potential difference.