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In-vitro binding of prostaglandin F-2 to uterine luminal proteins of pregnant and non-pregnant ewes

Summary. Pregnant and non-pregnant ewes were utilized to determine whether the presence of the embryo affected the binding of prostaglandin (PG) F-2 to a uterine luminal protein. The uterine horn adjacent to the corpus luteum was flushed on Day 13 of gestation or the oestrous cycle. Flushings were incubated with [³H]PGF-2 and subsequently eluted through a Sephadex column. Uterine luminal proteins of pregnant and non-pregnant ewes eluted with the void volume and failed to bind PGF-2.