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Changes in adenosine 5'-triphosphate, adenylate energy charge and adenosine 3'5'-cyclic monophosphate during the freezing of buffalo semen

Summary. In freshly ejaculated buffalo semen (N = 4) there were 24·61 ± 5·28 nmol ATP and 40·39 ± 5·94 nmol total adenylate/10⁸ spermatozoa, and 97·75 ± 7·06 pmol cAMP/10⁹ spermatozoa. The semen was frozen in 4 steps (I, dilution; II, cooling; III, glycerolization and equilibration; IV, freezing and thawing). Motility, ATP, total adenylate and cAMP were significantly lower after Step IV than after Step I.

Motility and ATP concentration were significantly correlated in egg-yolk—Tris (r = 0·530, P < 0·05), skim milk—egg yolk (r = 0·754, P < 0·01), egg yolk-citrate—glucose (r = 0·784, P < 0·01) and citric acid-whey (r = 0·551, P < 0·05), Cyclic AMP and motility in egg yolk-Tris were also correlated (r = 0·714, P < 0·01). The adenylate energy charge was stable in all 4 freezing steps.