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Summary. An antiserum raised against oestrone-3-glucuronide—BSA was used for the direct radioimmunoassay of oestrone sulphate in pig serum. The method has a minimum sensitivity of 0·2 ng/ml and is potentially an accurate pregnancy test in pigs. Serum oestrone sulphate concentrations were
0·5 ng/ml in non-pregnant cyclic sows and > 0·5 ng/ml in pregnant sows bled 26–29 days after service.
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