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Characterization of sperm surface and seminal plasma glycoproteins of the chimpanzee

Summary. Washed ejaculated chimpanzee spermatozoa and a 100 000 g supernatant of seminal plasma were subjected to radiolabelling by sequential treatment with galactose oxidase and sodium boro[³H]hydride or with sodium metaperiodate and NaB³H₄. Sperm surface glycoproteins and seminal plasma glycoproteins radiolabelled by these procedures were compared by SDS—polyacrylamide gel electrophoresis. Spermatozoa labelled by galactose oxidase treatment showed a single labelled macromolecular component of 37 000 whereas spermatozoa labelled by sodium metaperiodate—NaB³H₄ treatment showed incorporation into macromolecular components of 37 000 and 25 000 mol. wt. Seminal plasma radiolabelled by galactose oxidase—NaB³H₄ treatment contained labelled components of 47 000, 37 000, 19 000 and 12 000 mol. wt, whereas seminal plasma radiolabelled with sodium metaperiodate—NaB³H₄ contained macromolecular components of 47 000, 37 000, and 19 000 mol. wt.