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Enzyme-immunoassay for determination of progesterone in milk from cows

Summary. An enzyme-immunoassay using horseradish peroxidase as label is described. The assay has a sensitivity of 0·1–20 pmol, i.e. 0·03–0·63 ng and intra-and inter-assay coefficients of variation of < 10% when used for the determination of progesterone in samples of milk from cows. Comparison with a radio-immunoassay based on tritiated tracer showed excellent correlation (r = 0·98) for milk samples from cows during the oestrous cycle and early pregnancy.