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Summary. Oocytes were removed from follicles 5–30 mm in diameter. The germinal vesicle was present in 69·6% (23/33) of the oocytes at the start of culture, but after 20–24 and 40 h 70·5% (12/17) and 68·2% (43/63) of the oocytes were in metaphase I and metaphase II with first polar body extruded, respectively.
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