This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Cheng, K. W. Articles by Palmer, W. M. Search for Related Content PubMed PubMed Citation Articles by Cheng, K. W. Articles by Palmer, W. M.

Characterization of a radioimmunoassay for ovine FSH utilizing an anti-bovine FSH serum

Summary. The sensitivity of the assay, utilizing a bovine FSH antiserum and ¹²⁵I-labelled bovine FSH tracer, is 5 ng NIH-FSH-S6/ml, and cross-reactivities of other ovine pituitary glycoprotein and protein hormones (LH, TSH, growth hormone and prolactin) are less than 1·0%. Both pituitary and serum FSH of the sheep cross-react in a manner parallel with that of standard NIH-FSH-S6. Serum FSH concentrations in 4 ewes at various times after the onset of oestrus peaked more or less coincidently with the preovulatory increase of serum LH. The serum levels of FSH and LH did not correlate proportionally but fluctuated considerably in individual animals. The radioimmunoassay system appears to be sensitive and specific for measuring serum concentrations of FSH in rams and ewes in different physiological states.