This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Massip, A. Articles by Mulnard, J. Search for Related Content PubMed PubMed Citation Articles by Massip, A. Articles by Mulnard, J.

Time-lapse cinematographic analysis of hatching of normal and frozen—thawed cow blastocysts

Summary. This paper describes time-lapse cinematography on cattle blastocysts hatching from the zona in vitro. Four normal morulae were taken on Day 6 and cultured for 5 days. During this period 3 formed normal blastocysts of which 2 hatched from the zona without undergoing any contractions. Early blastocysts were taken on Day 7, frozen and stored at low temperature. After thawing they were cultured; only 1 of the 11 behaved like the normal embryos, and the others underwent contractions and failed to hatch. These contractions could be interpreted as a sign of an alteration of the trophoblastic structure and properties.

This article has been cited by other articles:

				G.V. Sireesha, R.W. Mason, M. Hassanein, S. Tonack, A. Navarrete Santos, B. Fischer, and P.B. Seshagiri Role of cathepsins in blastocyst hatching in the golden hamster Mol. Hum. Reprod., June 1, 2008; 14(6): 337 - 346. [Abstract] [Full Text] [PDF]

				D. S. Gonzales, B. D. Bavister, and S. A. Mese In Utero and In Vitro Proteinase Activity During the Mesocricetus auratus Embryo Zona Escape Time Window Biol Reprod, January 1, 2001; 64(1): 222 - 230. [Abstract] [Full Text]

				A. Mishra and P.B. Seshagiri Evidence for the involvement of a species-specific embryonic protease in zona escape of hamster blastocysts Mol. Hum. Reprod., November 1, 2000; 6(11): 1005 - 1012. [Abstract] [Full Text] [PDF]