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Concanavalin A binding capacity of preimplantation mouse embryos

Summary. Mouse embryos collected on Days 2 (2-cell) to 4 (early blastocyst) were treated with pronase to digest the zona pellucida and cultured at 37°C for 3 h. These embryos and untreated Day-5 late blastocysts were exposed to [³H]Con A at 25, 50, 100 or 200 µg/ml, 4°C for 30 min, washed and counted for radioactivity. Other groups of embryos were incubated with [³H]Con A in the presence of 0·06 M-methyl-D-mannoside for the detection of non-specific binding. Scatchard plot analysis showed that the total number of Con A molecules bound/embryo increased with development, from 12 x 10⁷ in 2-cell embryos to 19 x 10⁷ in early blastocysts and to 150 x 10⁷ in late blastocysts. The association constants decreased from 3 x 10⁶ M^–1 in 2-cell embryos to 0·83 x 10⁶ M^–1 in late blastocysts. The results suggest that the Con A binding sites (probably glycoproteins) on the embryo surface change quantitatively and qualitatively during preimplantation development.

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