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Within-subject variability of human semen in regard to sperm count, volume, total number of spermatozoa and length of abstinence

Summary. The within-subject variability of the semen sperm count (n), volume (v) and the total number of spermatozoa (N) was studied on 220 ejaculates from 36 normal subjects after an abstinence of 7 days or less. For each of the three variables, the within-subject standard deviation, , was practically proportional to the mean, µ; the common value of the coefficient of variation /µ for all subjects was very high: 0·39 for n, 0·28 for v and 0·55 for N. The 95% confidence intervals based on a single ejaculate were asymmetrical and very large, the lower and upper limits being respectively 0·5 x n and 2·3 x n; 0·7 x v and 1·8 x v; 0·4 x N and 2·9 x N. The three semen characteristics for a given subject were highly correlated with the length of abstinence: for an increase in abstinence of 1 day there were mean increments of 13 x 10⁶/ml for n, 0·4 ml for v, and 87 x 10⁶ for N.

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