This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Alemán, V. Articles by Delhumeau-Ongay, G. Search for Related Content PubMed Articles by Alemán, V. Articles by Delhumeau-Ongay, G.

A simple and rapid technique to isolate enriched populations of spermatocytes and spermatids from the immature rat testis

Summary. A technique was developed to obtain enriched populations of large numbers of primary spermatacytes (70–81%) and of spermatids (75%) from immature rat testes (21–23 and 38 days old respectively) in a simple and rapid fashion. The cells were nearly all viable and membrane preservation was good. The testicular cells were dispersed by a mild mechanical treatment combined with an incubation with purified collagenase, and the cell populations were separated by centrifugation in a discontinuous dextran gradient in a cell culture medium.