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A freeze-etch study of dense fibres in rat spermatozoa

Summary. The presence of oblique striations on the cortex of the dense fibres of the rat spermatozoon is confirmed; they have a main periodicity of approximately 40 nm and a secondary one of about 20 nm. Disruption of spermatozoa by Triton X-100 and dithiothreitol and fixation in glutaraldehyde do not affect the pattern. The dense fibre cortex appears to consist of globular substructure units with a diameter of about 19 nm.