This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Niwa, K. Articles by Iritani, A. Search for Related Content PubMed Articles by Niwa, K. Articles by Iritani, A.

Effect of various hexoses on sperm capacitation and penetration of rat eggs in vitro

Summary. Fertilization of rat eggs in vitro could not be achieved when epididymal spermatozoa were preincubated and eggs in clots incubated in a chemically defined medium without D-glucose. Very high proportions (84–100%) of eggs examined were undergoing fertilization when 2·78–8·34 mM-D-glucose were included in the medium. The substitution of D-fructose or D-galactose for D-glucose resulted in very poor penetration rates (0–4%), but D-mannose was effective for fertilization (59–99% penetration). Incubations for sperm capacitation and egg fertilization in different media containing the various hexoses showed that rat epididymal spermatozoa could be partly capacitated without hexose, that D-glucose, D-mannose or D-galactose but not D-fructose is effective for sperm capacitation and that only D-glucose and D-mannose supported penetration of eggs in vitro.

This article has been cited by other articles:

				G Kamp, H Schmidt, H Stypa, S Feiden, C Mahling, and G Wegener Regulatory properties of 6-phosphofructokinase and control of glycolysis in boar spermatozoa Reproduction, January 1, 2007; 133(1): 29 - 40. [Abstract] [Full Text] [PDF]

				T. Blasco, J. J. Aramayona, A. I. Alcalde, J. Catalan, M. Sarasa, and V. Sorribas Rat kidney MAP17 induces cotransport of Na-mannose and Na-glucose in Xenopus laevis oocytes Am J Physiol Renal Physiol, October 1, 2003; 285(4): F799 - F810. [Abstract] [Full Text] [PDF]

				F. Urner, G. Leppens-Luisier, and D. Sakkas Protein Tyrosine Phosphorylation in Sperm During Gamete Interaction in the Mouse: The Influence of Glucose Biol Reprod, May 1, 2001; 64(5): 1350 - 1357. [Abstract] [Full Text]

				F. Urner and D. Sakkas Characterization of Glycolysis and Pentose Phosphate Pathway Activity during Sperm Entry into the Mouse Oocyte Biol Reprod, April 1, 1999; 60(4): 973 - 978. [Abstract] [Full Text]

				F. Urner and D. Sakkas A Possible Role for the Pentose Phosphate Pathway of Spermatozoa inGamete Fusion in the Mouse Biol Reprod, March 1, 1999; 60(3): 733 - 739. [Abstract] [Full Text]