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The viability of deep-frozen cow embryos

Summary. Day 7 cow embryos were frozen in 1·5 M-DMSO in PBS at 0·3°C/min to –36°C and at 0·1°C/min between –36 and –60°C before being plunged directly into liquid nitrogen. They were subsequently thawed (rapidly to –50°C, at 4°C/min from –50 to –10°C, and rapidly again) to room temperature. Embryonic viability was tested by four different transfer techniques. Maximum pregnancy rate (8/12) was obtained with surgical transfer immediately after thawing and dilution of DMSO.

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