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Summary. Methods for collection, freeze preservation and artificial insemination of domestic cat semen were developed. Total sperm count and pre-freeze and post-thaw% motility were significantly greater (P < 0·05) for samples collected by an artificial vagina method than for those collected by electroejaculation. Although conception rate was low (10·6%), 6 pregnancies were achieved, 2 after hormonal induction of oestrus.
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