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Relationship between sperm concentration and the incidence of polyspermy in mouse embryos fertilized in vitro

Summary. A dose–response relationship between the incidence of polyspermy and sperm concentration was found by analysing chromosome preparations of first-cleavage mouse embryos derived from fertilizations in vitro with 3 concentrations of mouse spermatozoa (2 x 10⁴, 2 x 10 ⁶ and 1 x 10⁷/ml).