This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Trounson, A. O. Articles by Rowson, L. E. A. Search for Related Content PubMed Articles by Trounson, A. O. Articles by Rowson, L. E. A.

Fertilization and development capability of bovine follicular oocytes matured in vitro and in vivo and transferred to the oviducts of rabbits and cows

Summary. Follicular oocytes were cultured for 24 h in vitro or obtained 6–24 h after HCG injection from cows pretreated with PMSG, and transferred to the oviducts of oestrous rabbits or heifers, inseminated with bull spermatozoa, to determine their capability for normal development.

More than 65% of oocytes cultured in fetal calf serum, equilibrated with 5% CO₂ + 5% O₂ + 90% N₂, matured to metaphase II within 24 h. Fertilization was not obtained in the rabbit oviduct but about 8% of oocytes matured in vitro underwent parthenogenetic cleavage. Sperm penetration was observed in 49% of oocytes in the cow oviduct, but although 47% had undergone development 96 h after transfer few developed to morulae and blastocysts, confirming the impaired ability of oocytes matured in vitro, as assessed by nuclear change, to develop normally.

Oocytes obtained from heifers slaughtered 24 h after HCG developed normally when transferred to the oviduct of inseminated heifers. Of the oocytes with an activated cumulus, 39% developed to blastocysts and of 16 blastocysts transferred to suitable recipients, 13 developed to normal fetuses at 13–17 weeks gestation.

This article has been cited by other articles:

				G. Seidel Jr Superovulation and embryo transfer in cattle Science, January 23, 1981; 211(4480): 351 - 358. [Abstract] [PDF]