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Fluid reabsorption in the isolated duct of the rat cauda epididymidis

Summary. A technique is described for measurement of rate of fluid reabsorption in a segment of the rat cauda epididymidis in vitro. The basal rate was 2·63 ± 0·22 µl/cm²/30 min and was dependent on temperature and inhibited by 2,4-dinitrophenol, suggesting that fluid reabsorption is an energy-dependent process. About 50% of the fluid reabsorption was dependent on intraluminal sodium ions. This Na⁺-dependent component was inhibited by addition of amiloride to the intraluminal fluid and ouabain to the peritubular fluid.

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