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EFFECTS OF IN-VITRO TREATMENT OF BOAR SPERMATOZOA WITH TEPA ON THE FERTILIZATION AND DEVELOPMENT OF PIG EGGS

Animal Physiology and Genetics Institute, and Agricultural Environmental Quality Institute, U.S. Department of Agriculture, Beltsville, Maryland 20705, U.S.A.

(Received 20th May 1975)

Numerous studies have demonstrated the value of using heterospermic inseminations for comparing the fertilizing capacity of males (Edwards, 1955; Beatty, 1957, 1960; Beatty et al., 1969; Stewart et al., 1974; Overstreet & Adams, 1971; Martin & Reimers, 1973) and for assessing sperm treatments (Roche et al., 1968; Miller et al., 1969; Dziuk, 1970; O'Reilly et al., 1972). Overstreet & Adams (1971) and Bedford & Overstreet (1972) showed that X-irradiation of rabbit spermatozoa in vitro effectively 'marked' the sperm nucleus without affecting the fertilizing capacity. Ova fertilized by the 'marked' spermatozoa have retarded cleavage. The 'marked' spermatozoa were mixed with unmarked spermatozoa from the same ejaculate and used to test the comparative fertilizing capacity after different sperm treatments were superimposed on the two sperm populations.

Taber & Borkovec (1969)