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GROWTH OF OVARIAN FOLLICLES IN RATS IN THE ABSENCE OF GONADOTROPHINS

Department of Obstetrics and Gynaecology, Kobe University, School of Medicine, Kobe, Japan

(Received 5th May 1975)

Ovarian follicles grow and develop to the antral stage in rats and guinea-pigs, even after hypophysectomy (Dempsey, 1937; Paesi, 1949), indicating that gonadotrophic hormones are not essential for the early growth of follicles. Since these findings were based only on the histological appearance of the ovary after hypophysectomy, the present investigation was to determine the effect of hypophysectomy on the granulosa cells of growing follicles by using autoradiography after pulse-labelling with tritiated thymidine (Peters & Levy, 1966; Ryle, 1969a, b; How et al., 1970; Pedersen, 1970).

Female Sprague-Dawley rats were hypophysectomized at 22 days of age and kept in an air-conditioned room (22°C) under controlled lighting (12 hr light/ 24 hr) and with free access to food and water. Tritiated thymidine was injected intraperitoneally (2 µCi/g body wt) at 32 days of age, and

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