This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by KATZ, D. F. Articles by DOTT, H. M. Search for Related Content PubMed Articles by KATZ, D. F. Articles by DOTT, H. M.

METHODS OF MEASURING SWIMMING SPEED OF SPERMATOZOA

Summary.: Three basic approaches for determining the mean swimming speed of a suspension of microorganisms were compared, using bull and ram spermatozoa. Number fluctuation counting was performed automatically on a Quantimet 720 image analysing computer, the mean speed being obtained using 'probability after' statistics. The other two approaches were photomicrographic: number flux counting was performed on single photomicrographs; on the same photomicrographs, the mean speed was estimated from measurement of 'whole' and 'half' track lengths. These results were compared with each other and with the Quantimet results. The 'probability after' method was also compared, on additional samples, with cine-photomicrographic tracking. The mean speeds predicted by the 'probability after' method compared favourably with the other methods (range 68 µm/sec to 162 µm/sec). The results also suggested that, on single photomicrographs, measurement of 'half' track lengths or number flux counting were generally preferable to measurement of whole track lengths.

This article has been cited by other articles:

				J. Baumber and S. A. Meyers Hyperactivated Motility in Rhesus Macaque (Macaca mulatta) Spermatozoa J Androl, May 1, 2006; 27(3): 459 - 468. [Abstract] [Full Text] [PDF]

				J. Baumber and S. A. Meyers Hyperactivated Motility in Rhesus Macaque (Macaca mulatta) Spermatozoa J Androl, May 1, 2006; 27(3): 459 - 468. [Abstract] [Full Text] [PDF]