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LUTEOLYTIC FACTOR IN STALLION SEMEN

MANIKA WODZICKA-TOMASZEWSKA, A. OKÓLSKI, A. BIELASKI and W. BIELASKI

Agricultural Academy in Kraków, Institute of Applied Physiology of Animals, 30-059 Kraków, Al. Mickiewicza 24/28, Poland

(Received 13th December 1974)

Bielaski et al. (1974) demonstrated that oestrus and ovulation could be produced in anoestrous and dioestrous mares by the introduction of stallion semen into the uterus. Their results suggested that stallion semen may contain a luteolytic factor. Prostaglandin F₂ (PGF₂) is well known to be luteolytic in many species (McCracken et al., 1970). The aim of the present study was to compare the effects of PGF₂ and stallion semen on the CL.

Thirty-three pseudopregnant rabbits were assigned to Groups A, B and C (Table 1). Ovulation was induced with 100 i.u. HCG. Physiological saline, PGF₂ (trimethonium salt of PGF₂, `Prostin F_2alpha': Upjohn Company) and stallion semen, respectively, were introduced into the lumen of one uterine horn during laparotomy carried out on two different days