This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by WHITTINGHAM, D. G. Search for Related Content PubMed Articles by WHITTINGHAM, D. G.

SURVIVAL OF RAT EMBRYOS AFTER FREEZING AND THAWING

Physiological Laboratory, Downing Street, Cambridge CB2 3EG

(Received 13th December 1974)

Survival of mammalian embryos after storage at sub-zero temperatures was first achieved in the mouse in which all the preimplantation stages were shown to survive storage at –196°C when suitably cooled and thawed (Whittingham, Leibo & Mazur, 1972; Wilmut, 1972; Whittingham, 1974a). Similar procedures have demonstrated that blastocysts of cattle (Wilmut & Rowson, 1973), morulae and blastocysts of sheep (Willadsen, Polge, Rowson & Moor, 1974) and four-cell, eight-cell and morulae of rabbits (Bank & Maurer, 1973; Whittingham & Adams, 1974) will survive storage at – 196°C. This communication reports the survival of two-cell, four-cell and eight-cell rat embryos after storage at – 196°C for periods up to 3 months.

Superovulation of immature female rats, 26 to 28 days of age (CFHB random-bred strain, Carworth-Europe), with intraperitoneal injections of 25 i.u. PMSG and 10 i.u. HCG given 44 to 48

This article has been cited by other articles:

				M.-S. Han, K. Niwa, and M. Kasai In Vivo Development of Vitrified Rat Embryos: Effects of Timing and Sites of Transfer to Recipient Females Biol Reprod, February 1, 2004; 70(2): 425 - 429. [Abstract] [Full Text] [PDF]

				R. T. Pfaff, Y. Agca, J. Liu, E. J. Woods, A. T. Peter, and J. K. Critser Cryobiology of Rat Embryos I: Determination of Zygote Membrane Permeability Coefficients for Water and Cryoprotectants, Their Activation Energies, and the Development of Improved Cryopreservation Methods Biol Reprod, November 1, 2000; 63(5): 1294 - 1302. [Abstract] [Full Text]