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HORMONAL REGULATION OF PROTEINS IN THE UTERINE SECRETION OF OVARIECTOMIZED RATS AND THE IMPLICATIONS FOR IMPLANTATION AND EMBRYONIC DIAPAUSE

Summary.: Uterine secretions were obtained from long-term ovariectomized rats given progesterone for 10 days followed by oestrogen and progesterone. Analysis of the protein content was carried out using polyacrylamide disc gel electrophoresis at pH 8·9 and also of the proteinsodium dodecyl sulphate complexes. When progesterone was given alone, there was a lack of high molecular weight proteins. When oestradiol was also given, a protein (mol. wt 70,000) was detected within 1 hr. At 13 to 20 hr after oestrogen, there was a shift towards the secretion of high molecular weight proteins when the secretion profiles were similar to those of normal pregnant animals at Day 5, the day of implantation in the rat. The hormonal regulation of the secretion proteins is discussed. The physiological aspects of implantation in terms of zona lysis, embryonic diapause and the release from diapause after oestrogen treatment are discussed with reference to the changes in the intraluminal proteins.