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EMBRYOTOXIC EFFECT OF BULL SEMINAL RIBONUCLEASE AND TISSUE ABSORPTION STUDIES IN RATS

J. MATOUEK

Czechoslovak Academy of Sciences, Institute of Animal Physiology and Genetics, Libchov, Czechoslovakia

(Received 7th October 1974)

A protein substance responsible for embryotoxic and aspermatogenic effects in mice was isolated from bull seminal vesicle fluid by Dostál & Matouek (1973) and its ribonuclease activity was determined (Matouek, Pavlok, Dostál & Grozdanovi, 1973). This enzyme also caused disturbances of embryonic development in guinea-pigs and rabbits (Matouek, Fulka & Pavlok, 1973).

The present paper presents an account of the embryotoxic effect of the enzyme (AS RNase) in rats and the results of the study of absorption and binding of AS RNase by various embryonic and adult tissues.

Wistar rats weighing 250 to 270 g and 5 to 10 days pregnant were given a single subcutaneous injection of 40 mg pure AS RNase, together with 40, 80 or 120 mg partly purified ribonuclease designated AS RNase (BS) (Dostál & Matouek, 1973) diluted in

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