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Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu, Hawaii 96822, and Cancer Council and Electron Microscopy Laboratories, The Salk Institute for Biological Studies, San Diego, California 92112, U.S.A.
(Received 12th September 1974)
Mammalian eggs are surrounded by a moderately thick transparent coat, the zona pellucida, which is believed to be an important site of sperm recognition and of the block to polyspermy (Braden, Austin & David, 1954; Austin, 1961; Dickmann, 1962; Yanagimachi, 1964; Barros, 1968; Barros & Yanagimachi, 1972; Hanada & Chang, 1972; Hartmann, Gwatkin & Hutchison, 1972; Yanagimachi, 1972; Gwatkin, Williams, Hartmann & Kniazuk, 1973; Hartmann & Hutchison, 1974). Little is known of the molecular structure of the zona pellucida, but available evidence indicates that it is composed of glycoproteins or glycopeptides (Braden, 1952; Konecny, 1959; Stegner & Wartenberg, 1961; Lowenstein & Cohen, 1964; Jacoby, 1962; Soupart & Noyes, 1964; Fléchon, 1970; Inoue &
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