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THE EFFECT OF OESTROGEN AND PROGESTERONE ON THE INCORPORATION OF TRITIATED THYMIDINE IN MOUSE UTERI IN VITRO

Summary.: The incorporation of tritiated thymidine into the uteri of immature mice in organ culture was assessed by autoradiography and liquid scintillation spectrometry. Oestrogenand progesterone, alone or combined, stimulated an increase in the incorporation of tritiated thymidine, indicating that DNA synthesis is occurring in vitro. Progesterone alone stimulated incorporation of label into stromal and epithelial cells, but the combination of progesterone and oestrogen stimulated the labelling of epithelial cells more than of stromal cells.