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DIFFERENTIAL EFFECTS OF OESTROGEN ON THE UPTAKE OF NUCLEIC ACID PRECURSORS BY MOUSE BLASTOCYSTS IN VITRO

Autoradiographic studies indicate that mouse embryos undergoing delayed implantation show a marked increase in the incorporation of labelled amino acids or nucleic acid precursors 14 hr after oestrogen injection (Weitlauff & Greenwald, 1968) while 18 to 30 hr are needed for rats (Prasad, Dass & Mohla, 1968; Sanyal & Meyer, 1970; Jacobson, Sanyal & Meyer, 1970; Wu & Meyer, 1971). A direct effect of oestrogen on mammalian blastocysts cultured from the two-cell stage has also been observed. Although 17β-oestradiol at 25 mg/ml produces some degenerative effects in cultured mouse morulae and early blastocysts (Kirkpatrick, 1971), the hormone at 10^-10M, 10^-9M and 10^-5M stimulates uptake and incorporation of labelled amino acids in early mouse blastocysts cultured from the two-cell stage (Smith & Smith, 1971). These studies suggest that oestrogen has a direct effect on the blastocysts in vitro by stimulating the uptake and incorporation of