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ISOLATION AND SOME CHEMICAL PROPERTIES OF ASPERMATOGENIC SUBSTANCE FROM BULL SEMINAL VESICLE FLUID

J. DOSTÁL and J. MATOUEK

Summary.: Aspermatogenic substance (AS) was isolated from bull seminal vesicle fluid by precipitation with acetic acid and ammonium sulphate, and subjected to CM Sephadex C-50 and Sephadex G-100 column chromatography. The molecular weight of the monomer of AS was 22,000 and of the dimer was 44,000 as determined by gel filtration. The purity of the AS was controlled by disc electrophoresis in acrylamide gel, starch-gel electrophoresis, immunoelectrophoresis in agar gel, by peak homogeneity at the last step of separation and by the ultracentrifugation pattern. The N-terminal amino acid was lysine. The absorbance of 0·1% AS at 280 nm in pH 5·5 buffer was 0·510. The s value obtained for 0·5% AS solution was 2·7.

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