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SOME ASPECTS OF THE COMPOSITION OF BUFFALO SEMEN

An earlier report from this laboratory (Banerjee & Ganguli, 1971) was concerned with the electrophoretic and gel filtration behaviour of seminal plasma proteins in Zebu and buffalo bull semen. The chemical composition of Zebu semen has been reported by Mann (1964) and that of buffalo semen by Roy, Karnik, Lukuture, Bhattacharya & Bhattacharya (1950) and Roy, Pandey & Rawat (1960). The present work was undertaken in order to evaluate the nature of the composition of semen from Zebu and buffalo bulls maintained in our Institute's herd.

Semen samples were collected once a week, using the artificial vagina technique described by Walton (1945). Seminal plasma was separated by centrifuging whole semen at 2000 rev/min for 20 min.

Sperm counts were carried out by the method of Herman & Madden (1953). Fructose was estimated by the method of Roe (1934). Sialic acid, citric acid and ascorbic acid were determined by the methods