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THE CARBOHYDRATES OF BUFFALO AND GOAT SEMEN

Available studies on the carbohydrates of buffalo and goat semen are limited to estimations of fructose in whole semen. In order to have a complete picture of the `free' and `bound' carbohydrates present, the seminal plasma of these species was subjected to `extensive dialysis' as described by Mann & Rottenberg (1966). The results obtained are reported in this communication.

Semen was collected by artificial vagina and the plasma was separated from the spermatozoa in a clinical centrifuge. Pooled seminal plasma (5 to 10 ml) was dialysed against distilled water with five changes for 24 to 48 hr at 5° C. The two fractions, dialysable and non-dialysable, were taken to the original volume of the seminal plasma at 40° C under vacuum. The free sugars in the dialysable fraction were estimated after deproteinization with Ba(OH)₂ and ZnSO₄ followed by