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THE DEVELOPMENT OF MOUSE BLASTOCYSTS INJECTED WITH, OR CULTURED IN, TRYPAN BLUE SOLUTION

It is generally held that mammalian embryos are unaffected by most teratogens when these are given to the mother before implantation (Wilson, 1965). In an attempt to test this hypothesis, mouse blastocysts were either injected with, or cultured in, trypan blue solution and were then transferred to the uterus of a host animal for the remainder of gestation.

Blastocysts were obtained on the 4th day after superovulated black agouti (C3H/HeJ) mice were mated with Bagg albino (BALB/c) males. Trypan blue was dissolved in sterile balanced salt solution (Brinster, 1963) in concentrations of 0·10, 0·25 and 0·50%. For microinjection (Lin, 1966), blastocysts were deposited in a small drop of trypan blue solution in an `egg-well' which was then covered by mineral oil. Each blastocyst was immobilized by a holder-pipette (Plate 1) and trypan blue solution was